293T-EGFP cell line

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

293T-EGFP Order

Catalog#

YC-A007-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

293T-EGFP

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:293T			STR Info (Cell bank) Cell Line:HEK293T
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	8	9		8	9
D13S317	12	12		12	12
D7S820	11	11		11	11
D16S539	9	13		9	13
VWA	16	19		16	19
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the 293T cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a 293T-EGFP cell line that can maintain long-term, stable and high expression.

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